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human geckov2 crispr knockout pooled library  (Addgene inc)


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    Addgene inc human geckov2 crispr knockout pooled library
    Human Geckov2 Crispr Knockout Pooled Library, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmid+human+geckov2/TRBV4-1+(Plasmid+%23123411)/pm39232581-846-1-15
    Average 93 stars, based on 13 article reviews
    human geckov2 crispr knockout pooled library - by Bioz Stars, 2026-10
    93/100 stars

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    Plasmid Preparation:

    Article Title: Methods for selecting therapy for a cancer patient
    Article Snippet: Cells were transfected using LIPOFECTAMINE 2000 reagent and PLUS reagent (Invitrogen). .. For each flask, 4 mL of OptiMEM was mixed with 200 μL of PLUS reagent, 20 μg of LENTICRISPRV2 plasmid or pooled plasmid human GeCKOv2 (Genome-scale CRISPR Knock-Out) library, 15 μg psPAX2 (Addgene, Cambridge, Mass.) and 10 μg pMD2.G (Addgene). ..

    Article Title: Identification of essential genes for cancer immunotherapy
    Article Snippet: Cells were transfected using Lipofectamine 2000 and Plus reagent (Invitrogen). .. For each flask, 4 mL of OptiMEM was mixed with 200 μL of Plus reagent, 20 μg of lentiCRISPRv2 plasmid or pooled plasmid human GeCKOv2 (Genome-scale CRISPR Knock-Out) library, 15 μg psPAX2 (Addgene, Cambridge, MA) and 10 μg pMD2.G (Addgene). ..

    Article Title: A genome-scale crispr screen to identify essential genes for the effector function of cytotoxic T lymphocytes
    Article Snippet: Cells were transfected using Lipofectamine 2000 and Plus reagent (Invitrogen). .. For each flask, 4 mL of OptiMEM was mixed with 200 pL of Plus reagent, 20 pg of lentiCRISPRv2 plasmid or pooled plasmid human GeCKOV2 (Genome-scale CRISPR Knock-Out) library, 15 pg psPAX2 (Addgene, Cambridge, MA) and 10 pg pMD2.G (Addgene). ..

    CRISPR:

    Article Title: Methods for selecting therapy for a cancer patient
    Article Snippet: Cells were transfected using LIPOFECTAMINE 2000 reagent and PLUS reagent (Invitrogen). .. For each flask, 4 mL of OptiMEM was mixed with 200 μL of PLUS reagent, 20 μg of LENTICRISPRV2 plasmid or pooled plasmid human GeCKOv2 (Genome-scale CRISPR Knock-Out) library, 15 μg psPAX2 (Addgene, Cambridge, Mass.) and 10 μg pMD2.G (Addgene). ..

    Article Title: Identification of essential genes for cancer immunotherapy
    Article Snippet: Cells were transfected using Lipofectamine 2000 and Plus reagent (Invitrogen). .. For each flask, 4 mL of OptiMEM was mixed with 200 μL of Plus reagent, 20 μg of lentiCRISPRv2 plasmid or pooled plasmid human GeCKOv2 (Genome-scale CRISPR Knock-Out) library, 15 μg psPAX2 (Addgene, Cambridge, MA) and 10 μg pMD2.G (Addgene). ..

    Article Title: A genome-scale crispr screen to identify essential genes for the effector function of cytotoxic T lymphocytes
    Article Snippet: Cells were transfected using Lipofectamine 2000 and Plus reagent (Invitrogen). .. For each flask, 4 mL of OptiMEM was mixed with 200 pL of Plus reagent, 20 pg of lentiCRISPRv2 plasmid or pooled plasmid human GeCKOV2 (Genome-scale CRISPR Knock-Out) library, 15 pg psPAX2 (Addgene, Cambridge, MA) and 10 pg pMD2.G (Addgene). ..

    Knock-Out:

    Article Title: Methods for selecting therapy for a cancer patient
    Article Snippet: Cells were transfected using LIPOFECTAMINE 2000 reagent and PLUS reagent (Invitrogen). .. For each flask, 4 mL of OptiMEM was mixed with 200 μL of PLUS reagent, 20 μg of LENTICRISPRV2 plasmid or pooled plasmid human GeCKOv2 (Genome-scale CRISPR Knock-Out) library, 15 μg psPAX2 (Addgene, Cambridge, Mass.) and 10 μg pMD2.G (Addgene). ..

    Article Title: Identification of essential genes for cancer immunotherapy
    Article Snippet: Cells were transfected using Lipofectamine 2000 and Plus reagent (Invitrogen). .. For each flask, 4 mL of OptiMEM was mixed with 200 μL of Plus reagent, 20 μg of lentiCRISPRv2 plasmid or pooled plasmid human GeCKOv2 (Genome-scale CRISPR Knock-Out) library, 15 μg psPAX2 (Addgene, Cambridge, MA) and 10 μg pMD2.G (Addgene). ..

    Article Title: A genome-scale crispr screen to identify essential genes for the effector function of cytotoxic T lymphocytes
    Article Snippet: Cells were transfected using Lipofectamine 2000 and Plus reagent (Invitrogen). .. For each flask, 4 mL of OptiMEM was mixed with 200 pL of Plus reagent, 20 pg of lentiCRISPRv2 plasmid or pooled plasmid human GeCKOV2 (Genome-scale CRISPR Knock-Out) library, 15 pg psPAX2 (Addgene, Cambridge, MA) and 10 pg pMD2.G (Addgene). ..



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    Addgene inc geckov2 human whole genome library
    Fig. 1 Pooled CRISPR/Cas9 screening using the genome-wide single vector library <t>GeCKOv2</t> in MCF-7 cells. a Schematic outline of pooled CRISPR/ Cas9 screens under increased pharmacological pressure of either SI-12 or ICI to identify genetic vulnerabilities for drug combinations. b Molecular structures of SRC-3 inhibitors SI-2 and SI-12. c Timeline of the screens. Increased drug-pressure was applied on the cells and gDNA was collected at three- time points, which was subsequently amplified by PCR at the bar-coded regions and then subjected to NGS.
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    Fig. 1 Pooled CRISPR/Cas9 screening using the genome-wide single vector library GeCKOv2 in MCF-7 cells. a Schematic outline of pooled CRISPR/ Cas9 screens under increased pharmacological pressure of either SI-12 or ICI to identify genetic vulnerabilities for drug combinations. b Molecular structures of SRC-3 inhibitors SI-2 and SI-12. c Timeline of the screens. Increased drug-pressure was applied on the cells and gDNA was collected at three- time points, which was subsequently amplified by PCR at the bar-coded regions and then subjected to NGS.

    Journal: Communications biology

    Article Title: A genome-scale CRISPR Cas9 dropout screen identifies synthetically lethal targets in SRC-3 inhibited cancer cells.

    doi: 10.1038/s42003-021-01929-1

    Figure Lengend Snippet: Fig. 1 Pooled CRISPR/Cas9 screening using the genome-wide single vector library GeCKOv2 in MCF-7 cells. a Schematic outline of pooled CRISPR/ Cas9 screens under increased pharmacological pressure of either SI-12 or ICI to identify genetic vulnerabilities for drug combinations. b Molecular structures of SRC-3 inhibitors SI-2 and SI-12. c Timeline of the screens. Increased drug-pressure was applied on the cells and gDNA was collected at three- time points, which was subsequently amplified by PCR at the bar-coded regions and then subjected to NGS.

    Article Snippet: Lentiviruses were generated by co-transfecting either the pooled GeCKOv2 human whole-genome library (comprised of an equal contribution of the two sub-libraries—Lib.A and Lib.B) or pLJM1-EGFP (Addgene, cat. no. 19319 —a gift from David Sabatini), with packaging vectors psPAX2 (Addgene, #12260) and pMD2.G (Addgene, #12259)—both are gifts from Didier Trono.

    Techniques: CRISPR, Genome Wide, Plasmid Preparation